0
Chapter 5
Effects of Ultrasound Stimulation on Chondrocytes in Three-Dimensional Culture in Relation to the Production of Regenerative Cartilage Tissue

Excerpt

This chapter describes the effects of ultrasound stimulation on chondrocytes in three-dimensional culture in relation to the production of regenerative cartilage tissue, using collagen sponges as a carrier and supplementation with hyaluronic acid (used in the conservative treatment of osteoarthritis), and the mechanism of the adaptive response of chondrocytes to mechanical loading. It has been shown that cell proliferation and matrix production can be facilitated by considering the mechanical environment of the cultured chondrocytes and the mechanical properties of the scaffold structure used in the culture and of the stimulation used. On the basis of these results, it may be possible to establish the optimal conditions for mechanical stimulation, such as ultrasound frequency and intensity, and repetition frequency, for the production of regenerative cartilage tissue.

  • Abstract
  • 5.1 Introduction
  • 5.2 Functional Adaptation of Articular Cartilage to Mechanical Loading
  • 5.3 Use of Mechanical Stimulation for the Production of Regenerative Cartilage Tissue
  • 5.4 Materials and Methods for the Investigation of the Effects of Ultrasound Stimulation on Chondrocytes in Three-Dimensional Culture
  • 5.4.1 Cell Cultures
  • 5.4.2 Experimental Setup
  • 5.4.3 Experimental Methods
  • 5.4.4 Statistical Analyses
  • 5.5 Results and Discussions for the Investigation of the Effects of Ultrasound Stimulation on Chondrocytes in Three-Dimensional Culture
  • 5.5.1 Cell Proliferation
  • 5.5.2 Quantity Of Chondroitin Sulfates In Supernatants
  • 5.5.3 Histologic Examination
  • 5.5.4 Immunohistochemical Examination
  • 5.5.5 Type II Collagen Gene Expression
  • 5.6 Mechanism of Functional Adaptation of Chondrocytes to Mechanical Loading
  • 5.7 Summary
  • References

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In